FIGURE

Fig. 2

ID

ZDB-FIG-170914-53

Publication

Askary et al., 2017 - Genome-wide analysis of facial skeletal regionalization in zebrafish

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Fig. 2

Arch expression of predicted domain-specific genes. Whole-mount fluorescent in situ hybridizations for select genes were performed in sox10:GFPCAAX embryos at 36 hpf, with anti-GFP staining (green) showing CNCCs of the mandibular and hyoid arches. (A) foxf1, foxf2a, fzd9b, smad6a and skp2 are expressed in ventral domains of both arches, pitx1 only in the ventral mandibular arch, and sema3bl and twist1b in ventral and dorsal CNCCs. (B) fgfbp2a and shox are expressed in ventral-intermediate CNCCs, stmn1a in ventral-intermediate and dorsal CNCCs, tmem107I in ventral mandibular and posterior dorsal hyoid arches, and her6 in a more dorsal domain. (C) fsta, ctgfb, foxd1, emx2 and igfbp5b show specific intermediate domain expression; igfbp5b is also expressed in arch mesoderm. (D) cdh11, cd248a, kctd15a, pou3f3a, pou3f3b and postnb are expressed in dorsal CNCCs, sfrp2 in dorsal arch mesoderm, rassf10a in epithelia, and osr2 between the dorsal first arch and eye. Arrows point to expression in predicted domains, and arrowheads to other arch domains. Single optical sections are shown. Scale bar: 20 μm.

Expression Data

Genes:	cd248a cdh11 ccn2b EGFP emx2 fgfbp2a foxd1 foxf1 foxf2a fsta fzd9b her6 igfbp5b kctd15a osr2 pitx1 postnb pou3f3a pou3f3b rassf10a sema3bl sfrp2 shox skp2 smad6a stmn1a tmem107l twist1b
Fish:	el375Tg
Anatomical Terms:	pharyngeal arch 1 cranial neural crest pharyngeal arch 1 dorsal region pharyngeal arch 1 medial region pharyngeal arch 1 ventral region pharyngeal arch 1 ventral-most region pharyngeal arch 2 cranial neural crest pharyngeal arch 2 dorsal region pharyngeal arch 2 medial region pharyngeal arch 2 ventral region pharyngeal arch 2 ventral-most region
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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