Figure 5.

Ectopic Sema7A-GPI fails to guide sensory arbors from a distance.

(A) A diagrammatic overview depicts the generation of a transgenic animal that expresses the Sema7A-GPI protein ectopically under the control of a thermally inducible promoter. Larvae with ectopic myotomal-integration near the network of sensory arbors (red rectangle) were imaged to analyze arbor morphology. Uninjected, heat-shocked larvae were used as controls. (B) Uninjected and heat-shocked larva depicts normal arborization pattern of the sensory axons (magenta). (C) Injected and heat-shocked larvae robustly express the Sema7A-GPI in the myofibers (orange). Panels (i-iii) depict the ectopically expressing Sema7A-GPI myofibers that fail to attract sensory arbors toward itself. Scale bar, 20 µm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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