Figure 1—figure supplement 3.

Sema7A progressively enriches at the hair cell base with the sensory axons. (A) A micrograph at a nuclear plane of a 3 dpf neuromast depicts several actin-GFP (green) labeled hair cells. The lateral (white dashed line) and the basal (green dashed line) surfaces of three hair cells are outlined. (B) The td-Tomato-positive sensory arbors contact the basal regions (green dashed line) of three hair cells. (C) The Sema7A is enriched in the basal regions (green dashed line) of the hair cells that are in contact with the sensory arbors. (D, E) At the base of the hair cell, intensities of the td-Tomato-positive sensory arbors positively correlate with the Sema7A intensities. The results stem from 66 hair cells of 15 neuromasts from 2 dpf larvae and 40 hair cells of 11 neuromasts from 4 dpf larvae. Scale bars, 5 µm; au, arbitrary unit; ρ, Spearman’s correlation coefficient.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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