Figure 3—figure supplement 1.

Quantification of topological features across developmental stages and genotypes.

(A–C) The distribution of node degrees as a function of the height in hair cell clusters shows the difference in the network’s hierarchical organization between control and sema7a^-/- larvae. Nodes of low degree are commonly observed between genotypes across developmental stages (A, B), but their distribution along the cluster height became significantly different at 4 dpf (C), with nodes in control larvae localizing higher along the axis than in the mutants. In this and the following two panels, 33, 29, and 35 neuromasts were analyzed from 2 dpf, 3 dpf, and 4 dpf control larvae, respectively and 17, 53, and 27 neuromasts were analyzed from 2 dpf, 3 dpf, and 4 dpf sema7a^-/- mutant larvae, respectively (D, E) The correlations between the relative number of bare terminals and loops from control and sema7a^-/- neuromasts reveal dispersive distributions at both 2 dpf (D) and 3 dpf (E) stages, suggesting that the networks are undergoing reorganization and yet to adopt an ordered topology. (F, G) In the distribution of arbors between bare terminals and loops with respect to their contour lengths, mutants’ arbors display significantly higher propensity to remain as bare terminals compared to the controls at both 2 dpf (F) and 3 dpf (G). The samples were identical to those in the previous panel. Statistical analyses are displayed in Table 1.