Figure 3—figure supplement 2.

Quantification of topological features between control and sema7a^-/- larvae across developmental stages.

(A, B) Quantifications of the number of nodes per loop demonstrate a broader, but not significantly different, distribution in the mutants than the controls at both 2 dpf (A) and 3 dpf (B). In this and the following panels, 33 and 29 neuromasts were analyzed from 2 dpf and 3 dpf control larvae, respectively and 17 and 53 neuromasts were analyzed from 2 dpf and 3 dpf sema7a^-/- mutant larvae, respectively. (C, D) Quantifications of loop lengths from the same larvae indicate a wider, but not significantly different, distribution in the mutants than the controls at both 2 dpf (C) and 3 dpf (D). (E, F) The distributions of bare terminals between low- and high-curvature groups from the same larvae show higher occurrences of long, curved arbors in the control and short, linear arbors in the mutants at both 2 dpf (E) and 3 dpf (F) stages. Statistical analyses are displayed in Table 1.