The identification of crispants with the greatest photosensitivity can be obtained through a multiparametric analysis of their reaction to light flashes. (A): Bidimensional plot of the different variables selected as more relevant to perform the principal component analysis (PCA), with a vectorial representation. The selected variables are maximum velocity (mm/s), the number of angle turns, maximum acceleration (mm/s2), angular velocity (deg/s), mobility in the arena (%) and the cumulative duration (s) of three different mobility states, immobile, mobile and highly mobile. (B): PCA biplot comparing two main groups, the scrambled (in black) and the different studied crispants related to childhood epileptic genes (in blue). (C): The definition of two different populations depending on their activity through the calculation of Mahalanobis distance. The low activity group (in green) represents the population of analyzed larvae with a non-epileptic behavior; the high activity group (in orange) represents the population of analyzed larvae with an epileptic-like behavior in response to light flashes. (D): PCA biplot comparing the two main groups described through the Mahalanobis distance analysis, considering the different PCA variables. (E): A representation of the percentage of larvae classified in the epileptic-like population of the scrambled and all the selected crispants.
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