FIGURE

Fig. 5

ID

ZDB-FIG-190812-23

Publication

Elworthy et al., 2019 - The role of endothelial cilia in post-embryonic vascular development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Endothelial‐specific ablation of cilia does not perturb trunk or cerebral vascular development by 10 dpf. A: SPIM stack projections showing endothelial Clover expression in lateral views of the head, anterior trunk, posterior trunk, and tails of 10‐dpf Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (wt) (n = 9) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (ift54−/−;ubi:ift54) (n = 9) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (ift54−/−;ubi:ift54;kdrl:cre) (n = 4) fish. B: SPIM stack projections showing endothelial EGFP expression in dorsal views of the head of 10‐dpf Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:EGFP)y1^/+ (wt) (n = 4) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+; Tg(fli1a:EGFP)y1^/+ (ift54−/−;ubi:ift54) (n = 3) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898^/+; Tg(fli1a:EGFP)y1^/+ (ift54−/−;ubi:ift54;kdrl:cre) fish (n = 12). EGFP, enhanced green fluorescent protein; WT, wild‐type

Expression Data

Genes:	EGFP mClover
Fish:	mitfa^w2/w2; traf3ip1^tp49d/tp49d; s898Tg/+; sh467Tg/+; sh488Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; s898Tg/+; sh488Tg/+; y1Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh467Tg/+; sh488Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh488Tg/+; y1Tg/+
Anatomical Terms:	cranial vasculature trunk vasculature
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2; traf3ip1^tp49d/tp49d; s898Tg/+; sh467Tg/+; sh488Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; s898Tg/+; sh488Tg/+; y1Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh467Tg/+; sh488Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh488Tg/+; y1Tg/+
Observed In:	cranial vasculature trunk vasculature
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.