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Fig. 5

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ZDB-IMAGE-190812-22
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Figures for Elworthy et al., 2019
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Fig. 5

Endothelial‐specific ablation of cilia does not perturb trunk or cerebral vascular development by 10 dpf. A: SPIM stack projections showing endothelial Clover expression in lateral views of the head, anterior trunk, posterior trunk, and tails of 10‐dpf Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488/+;Tg(fli1a:LIFEACT‐clover)sh467/+ (wt) (n = 9) and ift54tp49Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488/+;Tg(fli1a:LIFEACT‐clover)sh467/+ (ift54−/−;ubi:ift54) (n = 9) and ift54tp49Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488/+;Tg(kdrl:cre)s898/+;Tg(fli1a:LIFEACT‐clover)sh467/+ (ift54−/−;ubi:ift54;kdrl:cre) (n = 4) fish. B: SPIM stack projections showing endothelial EGFP expression in dorsal views of the head of 10‐dpf Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488/+;Tg(fli1a:EGFP)y1/+ (wt) (n = 4) and ift54tp49Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488/+Tg(fli1a:EGFP)y1/+ (ift54−/−;ubi:ift54) (n = 3) and ift54tp49Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488/+;Tg(kdrl:cre)s898/+Tg(fli1a:EGFP)y1/+ (ift54−/−;ubi:ift54;kdrl:cre) fish (n = 12). EGFP, enhanced green fluorescent protein; WT, wild‐type

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