FIGURE

Fig. 4

ID

ZDB-FIG-190812-24

Publication

Elworthy et al., 2019 - The role of endothelial cilia in post-embryonic vascular development

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Fig. 4

Endothelial‐specific excision of the Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 rescue transgene. A: SPIM stack projections of lateral views of the head of 7‐dfp ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (ift54−/−; ubi:ift54; fli1:clover) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (itf54−/−; ubi:ift54; fli1:clover; kdrl:cre) fish showing endothelial Clover and faint mcherry expression. The position of the eye is labeled for orientation. Endothelial‐specific Cre‐mediated recombination of the Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 transgene is marked by mcherry expression. B: At 10 dpf, mcherry expression is more pronounced than at 7 dpf

Expression Data

Genes:	mCherry mClover
Fish:	mitfa^w2/w2; traf3ip1^tp49d/tp49d; s898Tg/+; sh467Tg/+; sh488Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh467Tg/+; sh488Tg/+
Anatomical Term:	eye endothelial cell
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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