FIGURE

Fig. 9

ID

ZDB-FIG-190812-18

Publication

Elworthy et al., 2019 - The role of endothelial cilia in post-embryonic vascular development

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Fig. 9

Endothelial‐specific excision of the Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488‐rescuing transgene causes no apparent further impairment to adult retina vasculature in ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 fish. Confocal stack projections showing endothelial EGFP expression in flat‐mount views of retina from sibling four‐month‐old Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:EGFP)y1^/+ (n = 5) (wt) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+; Tg(fli1a:EGFP)y1^/+ (n = 4) (ift54−/−;ubi:ift54) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898^/+; Tg(fli1a:EGFP)y1^/+ (n = 5) (ift54−/−;ubi:ift54;kdrl:cre) fish. The yellow boxes indicate the region of retina enlarged below. The Tg(kdrl:cre)s898^/+ caused no apparent further impairment to the vasculature in the fish with partial transgenic rescue of ift54^tp49. EGFP, enhanced green fluorescent protein; WT, wild‐type. Scale bars = 300 μm

Expression Data

Gene:	EGFP
Fish:	mitfa^w2/w2; traf3ip1^tp49d/tp49d; s898Tg/+; sh488Tg/+; y1Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh488Tg/+; y1Tg/+
Anatomical Term:	retina endothelial cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2; traf3ip1^tp49d/tp49d; s898Tg/+; sh488Tg/+; y1Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh488Tg/+; y1Tg/+
Observed In:	retina angiogenic sprout retina blood vessel
Stage:	Adult

Phenotype Detail

Acknowledgments

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