FIGURE

Fig. 8

ID

ZDB-FIG-190812-19

Publication

Elworthy et al., 2019 - The role of endothelial cilia in post-embryonic vascular development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

Endothelial‐specific excision of the Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488‐rescuing transgene causes no apparent further impairment to adult vasculature in ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 fish. A: Five‐month‐old ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+ (ift54−/−;ubi:ift54) (n = 24) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+; Tg(kdrl:cre)s898^/+ (ift54−/−;ubi:ift54;kdrl:cre) (n = 26) fish. The Tg(kdrl:cre)s898^/+ caused no apparent further impairment to fish with partial transgenic rescue of ift54^tp49. B: Confocal stack projections showing endothelial EGFP expression in flat‐mount views of swim bladder posterior chamber, pectoral fin, and 4 day‐postamputation regenerated caudal fin from sibling four‐month‐old Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:EGFP)y1^/+ (n = 5) (wt) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+; Tg(fli1a:EGFP)y1^/+ (representative of four animals analyzed) (ift54−/−;ubi:ift54) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898^/+; Tg(fli1a:EGFP)y1^/+ (n = 5) (ift54−/−;ubi:ift54;kdrl:cre) fish. Yellow lines indicate approximate position of original fin amputation. EGFP, enhanced green fluorescent protein; WT, wild‐type. Scale bars = 300 μm

Expression Data

Gene:	EGFP
Fish:	mitfa^w2/w2; traf3ip1^tp49d/tp49d; s898Tg/+; sh488Tg/+; y1Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh488Tg/+; y1Tg/+
Condition:	amputation: caudal fin
Anatomical Terms:	pectoral fin endothelial cell regenerating fin endothelial cell swim bladder endothelial cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh467Tg/+; sh488Tg/+ mitfa^w2/w2; traf3ip1^tp49d/tp49d; sh488Tg
Observed In:	vertebral column
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.