FIGURE

Fig. 6

ID
ZDB-FIG-190812-22
Publication
Elworthy et al., 2019 - The role of endothelial cilia in post-embryonic vascular development
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Fig. 6

Endothelial‐specific excision of the Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488transgene causes no apparent further impairment to secondary caudal fin vasculature in ift54tp49Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 fish. A: SPIM stack projections of endothelial cells marked with Clover expression in the caudal fin of 10‐dpf and 14‐dpf Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 /+;Tg(kdrl:cre)s898 /+;Tg(fli1a:LIFEACT‐clover)sh467 /+ fish, showing endothelial‐specific Clover and myr‐mcherry expression. B: SPIM stack projections of endothelial cells marked with Clover in the caudal fin of 14‐dpf ift54 tp49Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 /+;Tg(fli1a:LIFEACT‐clover)sh467 /+ (ift54−/−; ubi:ift54) and ift54 tp49Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 /+;Tg(kdrl:cre)s898/+;Tg(fli1a:LIFEACT‐clover)sh467 /+ (ift54−/−; ubi:ift54; kdrl:cre). The secondary caudal fin vasculature forms in the absence of endothelial ift54 function. C: SPIM stack projections of endothelial cells marked with Clover in the caudal fin of 13‐dpf ift54 tp49Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 /+;Tg(fli1a:LIFEACT‐clover)sh467 /+ (ift54−/−; ubi:ift54) and ift54 tp49Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 /+;Tg(kdrl:cre)s898 /+;Tg(fli1a:LIFEACT‐clover)sh467 /+ (ift54−/−; ubi:ift54; kdrl:cre) fish. The Tg(kdrl:cre)s898 /+ caused no apparent impairment to the developing fin vascular bed in the fish with partial transgenic rescue of ift54 tp49. Chart shows caudal fin vessel bed relative areas measured from SPIM images. Overlay shows median and interquartile range. Two‐tailed Mann‐Whitney test P = 0.7223

Expression Data
Genes:
Fish:
Anatomical Term:
Stage Range: Days 7-13 to Days 14-20

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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