ZFIN Image: Elworthy et al., 2019, Fig. 8

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Figure Caption

Fig. 8

Endothelial‐specific excision of the Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488‐rescuing transgene causes no apparent further impairment to adult vasculature in ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 fish. A: Five‐month‐old ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+ (ift54−/−;ubi:ift54) (n = 24) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+; Tg(kdrl:cre)s898^/+ (ift54−/−;ubi:ift54;kdrl:cre) (n = 26) fish. The Tg(kdrl:cre)s898^/+ caused no apparent further impairment to fish with partial transgenic rescue of ift54^tp49. B: Confocal stack projections showing endothelial EGFP expression in flat‐mount views of swim bladder posterior chamber, pectoral fin, and 4 day‐postamputation regenerated caudal fin from sibling four‐month‐old Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:EGFP)y1^/+ (n = 5) (wt) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+; Tg(fli1a:EGFP)y1^/+ (representative of four animals analyzed) (ift54−/−;ubi:ift54) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898^/+; Tg(fli1a:EGFP)y1^/+ (n = 5) (ift54−/−;ubi:ift54;kdrl:cre) fish. Yellow lines indicate approximate position of original fin amputation. EGFP, enhanced green fluorescent protein; WT, wild‐type. Scale bars = 300 μm

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Acknowledgments

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