FIGURE

Figure 2—figure supplement 1.

ID

ZDB-FIG-210204-92

Publication

Parab et al., 2021 - Endothelial cell type-specific molecular requirements for angiogenesis drive fenestrated vessel development in the brain

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Figure 2—figure supplement 1.

<italic>vegfab</italic> genetically interacts with <italic>vegfd</italic> in mCP vascular development.

(A) Schematic representation of the dorsal view of WT cranial vasculature, illustrating the mCP vasculature (DLV, PCeV) and the neighboring cranial vessels (MsV, MCeV). (B) Percentage of 96 hpf fish of indicated genotype with and without the DLV. vegfab^-/-;vegfd^-/- larvae displayed a significantly higher penetrance of the ‘No DLV’ phenotype (97%) than vegfab^-/- (69%) and vegfd^-/- (12.5%) larvae, showing that vegfab and vegfd genetically interacts in DLV formation (n = 24 for vegfd^-/-, n = 36 for vegfab^-/-, n = 41 for vegfab^-/-;vegfd^+/-, and n = 29 for vegfab^-/-;vegfd^-/- fish). (C) Quantification of PCeV, MCeV, and MsV formation at 96 hpf. PCeV formation was severely compromised in vegfab^-/-;vegfd^-/- larvae compared to the corresponding single mutants. At this stage, MsV, but not MCeV, formation was mildly affected in vegfab;vegfd double mutants compared to the corresponding single mutants. Data are means ± SD.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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