FIGURE

Figure 6—figure supplement 1.

ID

ZDB-FIG-210204-101

Publication

Parab et al., 2021 - Endothelial cell type-specific molecular requirements for angiogenesis drive fenestrated vessel development in the brain

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Figure 6—figure supplement 1.

Validation and expression analyses of distinct <italic>vegf</italic> BAC transgenic lines.

(A–F’) Endogenous mRNA transcripts of vegfab (A and A’), vegfc (C and C’), and vegfd (E and E’) at the indicated embryonic stages were detected by in situ hybridization. Lateral views of the EGFP expression patterns driven by the Tg(UAS:EGFP) embryo carrying the TgBAC(vegfab:gal4ff) (B and B’), TgBAC(vegfc:gal4ff) (D and D’), or TgBAC(vegfd:gal4ff) (F and F’) transgene. The EGFP expression patterns driven by each of the BAC transgenic lines closely matched those of the corresponding endogenous mRNA. (G–J) Dorsal views of 48 hpf Tg(kdrl:ras-mCherry) embryos with no additional gene (G) or carrying the TgBAC(vegfab:EGFP) (H), TgBAC(vegc:EGFP) (I), or TgBAC(vegd:EGFP) (J) transgene. No detectable auto-fluorescence was observed in the control embryo (G) under these microscopy settings. TgBAC(vegfab:EGFP), TgBAC(vegfc:EGFP), and TgBAC(vegfd:EGFP) expression was observed in dorsal meningeal surfaces and distinct cell types. (K and L) Lateral (K) and dorsal (L) views of 72 hpf TgBAC(vegfab:EGFP);Tg(kdrl:ras-mCherry) larvae, showing the TgBAC(vegfab:EGFP) expression in the DMJ and mCP. TgBAC(vegfab:EGFP) expression was not observed in the DLV. Scale bars: 200 μm in (F); 100 μm in (F’); 50 μm in (J), (K), (L).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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