Figure 6—figure supplement 5.

(A) Schematic of the ntla:EGFP construct used for injection experiments (B–E"). (B–E") Dorsal views of 48 hpf Tg(ntla:NTR) (B–C") and TgBAC(vegfc:NTR) (D–E") heads of embryos that were injected with the ntla:EGFP construct at the one-cell stage. Magnified images of the boxed area in (B) and (D) are shown in (C–C") and (E–E"), respectively. Most of the EGFP⁺ cells (C and E) were co-localized with NTR-mCherry⁺ meningeal cells (C’ and E’) in Tg(ntla:NTR) (C–C") and TgBAC(vegfc:NTR) (E–E") embryos. Yellow arrows point to the co-localized cells (n = 5 embryos, 71 co-localized cells observed for Tg(ntla:NTR); n = 3 embryos, 10 co-localized cells observed for TgBAC(vegfc:NTR)). (F–H) Dorsal magnified views of 48 hpf Tg(ntla:Gal4-VP16);Tg(UAS:EGFP-CAAX), abbreviated Tg(ntla:EGFP), TgBAC(vegfd:EGFP), and TgBAC(vegfab:EGFP) embryonic heads near the DMJ in the dorsal surfaces of the brain meninges. Close similarity in the morphology of the EGFP⁺ meningeal cells marked by each of the lines was observed. Scale bars: 50 μm in (B–E"); 25 μm in (H).