Figure 6—figure supplement 4.

Co-localization analyses in <italic>TgBAC(vegfab:NTR)</italic>, <italic>TgBAC(vegfc:NTR)</italic>, and <italic>TgBAC(vegfd:NTR)</italic> embryos carrying the <italic xmlns:xlink='http://www.w3.org/1999/xlink'>Tg(mpeg1.1:Dendra2)</italic> transgene.

(A–A") Confocal maximum projection images of the dorsal views of a 48 hpf Tg(mpeg1.1:Dendra2);TgBAC(vegfab:NTR) embryo, showing non-overlapping distribution of EGFP+ and NTR-mCherry+ meningeal cells (n = 16 embryos; 89 Tg(mpeg1.1:Dendra2)+ cells examined; 0 cells co-localized with TgBAC(vegfab:NTR)+ cells). (B–B") Confocal maximum projection images of the dorsal views of a 48 hpf Tg(mpeg1.1:Dendra2);TgBAC(vegfc:NTR) embryo, showing non-overlapping distribution of EGFP+ and NTR-mCherry+ meningeal cells (n = 17 embryos; 95 Tg(mpeg1.1:Dendra2)+ cells examined; 0 cells co-localized with TgBAC(vegfc:NTR)+ cells). (C–C") Confocal maximum projection images of the dorsal views of a 48 hpf Tg(mpeg1.1:Dendra2);TgBAC(vegfd:NTR) embryo, showing non-overlapping distribution of EGFP+ and NTR-mCherry+ meningeal cells (n = 8 embryos; 39 Tg(mpeg1.1:Dendra2)+ cells examined; 0 cells co-localized with TgBAC(vegfd:NTR)+ cells). Scale bar: 50 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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