Figure 1—figure supplement 3.

(A) Experimental time course of the heat-shock treatments. Tg(hsp70l:sflt1), Tg(hsp70l:sflt4), and their control siblings were subjected to a heat-shock at 41, 49, 57, and 65 hpf and analyzed at 72 hpf. (B–G) Dorsal views of the cranial vasculature of 72 hpf Tg(kdrl:EGFP) (B and E), Tg(hsp70l:sflt1);Tg(kdrl:EGFP) (C and F), and Tg(hsp70l:sflt4);Tg(kdrl:EGFP) (D and G) larvae after treatment with (E–G) or without (B–D) multiple heatshocks. (H–K) Percentage of the 72 hpf fish with and without the DLV after the indicated treatment (H and I). Quantification of the DLV lengths of fish subjected to the indicated treatment (J and K). The results involving sFlt1 overexpression are shown in (H and J) and those involving sFlt4 overexpression are shown in (I and K). The results of their control siblings with and without the heatshocks are shared in the graphs for both groups. Heatshock-induced overexpression of sFlt4 led to a significantly increased percentage of the ‘No DLV’ phenotype (33%) compared to the other control groups (I), while DLV lengths were significantly shorter in the fish subjected to heatshock-induced overexpression of sFlt1 compared to the other control groups (J). The number of the animals examined is listed in the graph. Data are means ± SD. Scale bar: 50 µm.