Fig. S6

The specific role of integrin pathway for HSPC expansion, related to figure 5.

A. The Venn diagram showing the overlapped receptors from the comprehensive analysis of three sequencing methods.

B. The Venn diagram showing the overlapped ligands from the comprehensive analysis of three sequencing methods.

C. WISH showing the expression of ctgfa and itgb2 at 2 dpf, 3 dpf and 4 dpf in zebrafish CHT region. 

D. The validation of ctgfa and itgb2 morpholino efficiency by fusion GFP plasmid injection.

E. WISH showing the expression of runx1 in control embryos, itgb2 morphants and ctgfa morphants at 30 hpf.

F. Confocal imaging of the transgenic line (CD41:GFP/gata1;dsRed) showing hematopoietic cells in the CHT region in control embryos, itgb2 morphants and ctgfa morphants at 3.5 dpf. 

G. The quantification data of CD41:GFP cells number in (F). Data are shown as means +- SEM. n=4, ns (no signifigant) P>0.05, *P<0,05.

H. WISH the expression of showing cmyb, gatal, coro1a and mfap4 in control embryos, ctgfa morphants and itgb2 morphants at 4.5 dpf.

I. qPCR results showing the expression of cmyb, pu. 1 and gatal in control embryos, ctgfa morphants and itgb2 morphants at 4 dpf. Data are shown as means +- SEM. n=3, **P<0.01, ***P<0.001.

J. WISH data showing the expression of ctgfa in GFP+ and GFP- embryos at 3.5 dpf after heat shocking transgenic (hsp701:ccn2a-2A-EGFP) embryos.

K. WISH results displaying the expression of cmyb at 4dpf in control embryos, ctgfa morphants, ctgfa MO injected hsp701:ccn2a-2A-EGFP+ embryos.

L. The identification of itgb2 mutants by sequancing analysis of genomic DNA with seven base pairs deletion.

M. The illustration showing the normal protein structure in wild-type embryos and the truncated protein structure in itgb2 mutants.

N. WISH data showing the expression of cmyb in sibling and itgb2 mutants 4dpf.