FIGURE

Fig. 5

ID

ZDB-FIG-181024-9

Publication

Xu et al., 2018 - Fox proteins are modular competency factors for facial cartilage and tooth specification

Other Figures

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Fig. 5

Fox genes are required for chondrogenic gene expression downstream of Sox9a. (A) Fluorescence in situ hybridizations show that barx1⁺ pre-cartilage condensations of the first two arches are unaltered in foxc1a; foxc1b double mutants (c1a^−/−; c1b^−/−) and foxf1; foxf2a; foxf2b triple mutants (f1^−/−; f2a^−/−; f2b^−/−) compared with sibling controls. (B,C) Schematic of the cartilaginous facial skeleton shows regions examined by fluorescence in situ hybridization. Maximum intensity projections for foxc1a; foxc1b mutants at 56 hpf (B) and representative sections for foxf1; foxf2a; foxf2b mutants at 72 hpf (C) show expression of the chondrogenic genes col2a1a, matn1, matn4 or acana (green) relative to sox9a (red). Arrows indicate regions in which sox9a⁺ cells have reduced chondrogenic gene expression relative to sibling controls. Numbers denote proportions of embryos with displayed patterns. Scale bars: 50 μm.

Expression Data

Genes:	acana barx1 col2a1a matn1 matn4 sox9a
Fish:	WT foxc1a^el543/+; foxc1b^el620/el620 foxc1a^el543/el543; foxc1b^el620/el620 foxf1^el658/el658; foxf2a^el616/el616; foxf2b^el621/+ foxf1^el658/el658; foxf2a^el616/el616; foxf2b^el621/el621
Anatomical Terms:	hyosymplectic cartilage Meckel's cartilage palatoquadrate cartilage pharyngeal arch 1 pharyngeal arch 2
Stage Range:	Prim-25 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	foxc1a^el543/el543; foxc1b^el620/el620 foxf1^el658/el658; foxf2a^el616/el616; foxf2b^el621/el621
Observed In:	hyosymplectic cartilage Meckel's cartilage palatoquadrate cartilage
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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