FIGURE

Fig. 6

ID

ZDB-FIG-171201-33

Publication

Khatri et al., 2016 - Down-regulation of coasy, the gene associated with NBIA-VI, reduces Bmp signaling, perturbs dorso-ventral patterning and alters neuronal development in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Effects of coasy-MO microinjection in the transgenic line Tg(fli1a:EGFP- gata1a:DsRed).

Lateral views of the trunk/tail regions of Tg(fli1a:EGFP-gata1a:DsRed) embryos injected with either ST-MO (a,d) or coasy-MO (b,c,e,f) at 1.2 pmol/embryo, eventually exposed to CoA in fish water (c,f) and analyzed at 40 hpf. fli1a (EGFP) marks vascular development, and gata-1 (DsRed) marks red blood cells. In coasy morphants compromission of the vascular system and particularly of the intersegmental vessels is evident (c,d) and associated with reduction in the number of blood cell (e,f). Results are from one representative experiment with at least 80 embryos out of three independent replicates.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sd2Tg; y1Tg + MO1-coasy
Condition:	chemical treatment by environment: coenzyme A
Knockdown Reagent:	MO1-coasy
Observed In:	intersegmental vessel nucleate erythrocyte vasculature development
Stage:	Prim-25

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.