FIGURE

Fig. S2

ID

ZDB-FIG-171201-36

Publication

Khatri et al., 2016 - Down-regulation of coasy, the gene associated with NBIA-VI, reduces Bmp signaling, perturbs dorso-ventral patterning and alters neuronal development in zebrafish

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Fig. S2

Expression pattern of coasy transcript during zebrafish development

Spatiotemporal analysis of coasy expression by WISH using a coasy-specific probe. WISH was performed from 0.2 hpf (1-2 cells) to 36 hpf with a coasy-specific antisense probe. (A) Dorsal view, four cells stage; (B) lateral view, sphere stage; (C) dorsal view, germ ring. (D-G) 20, 24, 36 hpf lateral views; (G) 36 hpf lateral view, magnification of somites. For each stage, at least 30 embryos were analyzed in two independent experiments. mhb, midbrain-hindbrain boundary; h, hindbrain; hbv, hindbrain ventricle; tg, tegmentum; s, somites. A digoxigenin-labelled, coasy sense probe was synthezised as described in Material and Methods and applied for in situ hybridization with embryos at different developmental stages (H-J). No specific labelling was evident at any stage. Rapresentative images from one experiment performed with at least 30 embryos and repeated twice.

Expression Data

Gene:	coasy
Fish:	AB
Anatomical Terms:	central nervous system hindbrain midbrain hindbrain boundary somite tegmentum whole organism
Stage Range:	4-cell to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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