FIGURE

Fig. 6

ID

ZDB-FIG-160224-6

Publication

Ulrich et al., 2016 - Reck enables cerebrovascular development by promoting canonical Wnt signaling

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Fig. 6

The cerebrovascular expression of the transgenic reporter of canonical Wnt signaling Tg(7xTCF-Xla.Siam:GFP)^ia4 is specifically lost in reck ^y72 mutant embryos. (A-N′′) Confocal images (anterior, left) of 36 and 48hpf Hbs from WT and reck ^y72. Views: lateral (A-H′′; dorsal, up); dorsal (I-N′′; right side, up). Endothelium, red [Tg(kdrl:RFP)^s896]; Wnt reporter, green [Tg(7xTCF-Xla.Siam:GFP)^ia4]. In the WT, Wnt reporter expression highlights the PHBCs (A-D′′), CtAs (A-D′′,I-K′′) and additional non-vascular tissues. However, in reck ^y72 mutants, expression of the Wnt reporter is undetectable in PHBCs (E-H′′,L-N′′) and occasional CtAs (N-N′′), but is present elsewhere. Detail zooms: Regions in white dashed boxes (A,E,I,L) shown in C-C′′,G-G′′,K-K′′ and N-N′′, respectively; regions in yellow dashed boxes (C,G) shown in D-D′′ and H-H′′, respectively. See also Fig. 8.

Expression Data

Genes:	GFP mCherry
Fish:	ia4Tg; s896Tg reck^y72/y72; ia4Tg; s896Tg
Anatomical Terms:	blood vessel endothelial cell central artery primordial hindbrain channel
Stage Range:	Prim-25 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	reck^y72/y72; ia4Tg; s896Tg
Observed In:	brain vasculature central artery central artery canonical Wnt signaling pathway primordial hindbrain channel primordial hindbrain channel canonical Wnt signaling pathway
Stage Range:	Prim-25 to Long-pec

Phenotype Detail

Acknowledgments

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