FIGURE

Fig. S10

ID

ZDB-FIG-160224-16

Publication

Ulrich et al., 2016 - Reck enables cerebrovascular development by promoting canonical Wnt signaling

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Fig. S10

Chemical activation of canonical Wnt signaling with the GSK3b inhibitors CHIR99021 and LiCl fails to rescue the DRG and CtA deficits of reck^y72 mutants. (A-H) Confocal lateral images at 72 hpf. Anterior, left; dorsal, up. Scale Bars: 100 µm. Genotypes and treatments as indicated. (A-D) DRG (white circles, green HuC immunofluorescence) and vasculature (Tg(kdrl:HsHRAS-mCherry)^s896; red) in the trunk. (E-H) Hb vasculature (Tg(kdrl:HsHRAS-mCherry)^s896; red). Central Arteries (CtA; white arrowheads). (I) Quantification of DRG abundance in WT and reck^y722 mutants treated with DMSO vehicle, CHIR99021 and LiCl. DRG abundance was scored by imaging a region of the anterior trunk at the level of the yolk extension spanning 3-4 Se vessels (in WT animals there is one DRG per Se vessel). Thus, the percentages were calculated from the ratio of DRG found/DRG expected.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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