FIGURE

Fig. 1

ID

ZDB-FIG-160224-1

Publication

Ulrich et al., 2016 - Reck enables cerebrovascular development by promoting canonical Wnt signaling

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Fig. 1

nft ^y72 mutant embryos lack intracerebral blood vessels and DRG but have normal body morphology. Confocal (A-H) and bright-field (I-L) lateral images. Anterior, left; dorsal, up. A,B,E,G: 72 hpf; C,D: 48 hpf; F,H: 96 hpf; I-L: 60 hpf. (A,B) Central Arteries (CtAs) are found in WT (A) (white arrowheads) but are missing in nft ^y72 mutants (B); the other head vessels are present in nft ^y72. Blood vessels [Tg(kdrl:RFP)^s896], red. WT (C) and nft ^y72 mutants (D) show identical trunk vascular patterns. Endothelium [Tg(fli1a:eGFP)^y1], green; somite boundaries, blue (Zygmunt et al., 2011). DRG (yellow circles; red HuC immunofluorescence) are present in WT (E) but absent in nft ^y72 mutants (G). (F,H) Blood vessels are green [Tg(fli1a:eGFP)^y1] and red [Tg(kdrl:RFP)^s896]; lymphatics (asterisks) are green only [Tg(fli1a:eGFP)^y1]. (J,L) Close-ups of head in I,K. MtA, metencephalic artery; PHBC, primordial hindbrain channel; DLAV, dorsal longitudinal anastomotic vessel; Se, intersegmental vessel; PAC, parachordal chain; Fb, forebrain; Mb, midbrain; Hb, hindbrain. Scale bars: 100µm in A,B, 50µm in C-E,G, 25µm in F,H and 200µm in I-L. See also Fig. S1, Movies 1-14 and Table S1.

Expression Data

Genes:	EGFP mCherry
Antibody:	Ab1-elavl
Fish:	WT reck^y72/y72 s896Tg; y1Tg reck^y72/y72; s896Tg; y1Tg
Anatomical Terms:	blood vessel endothelial cell central artery dorsal aorta dorsal longitudinal anastomotic vessel dorsal root ganglion lymphangioblast cord lymph vessel endothelium metencephalic artery posterior cardinal vein primordial hindbrain channel spinal cord thoracic duct lymph vessel endothelium
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	reck^y72/y72 reck^y72/y72; s896Tg; y1Tg
Observed In:	brain vasculature central artery central artery vasculature development dorsal root ganglion dorsal root ganglion morphogenesis head heart contraction peripheral nervous system thoracic duct lymph vessel development trunk vasculature
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

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