ZFIN Image: Ulrich et al., 2016, Fig. 6

Image

Figure Caption

Fig. 6

The cerebrovascular expression of the transgenic reporter of canonical Wnt signaling Tg(7xTCF-Xla.Siam:GFP)^ia4 is specifically lost in reck ^y72 mutant embryos. (A-N′′) Confocal images (anterior, left) of 36 and 48hpf Hbs from WT and reck ^y72. Views: lateral (A-H′′; dorsal, up); dorsal (I-N′′; right side, up). Endothelium, red [Tg(kdrl:RFP)^s896]; Wnt reporter, green [Tg(7xTCF-Xla.Siam:GFP)^ia4]. In the WT, Wnt reporter expression highlights the PHBCs (A-D′′), CtAs (A-D′′,I-K′′) and additional non-vascular tissues. However, in reck ^y72 mutants, expression of the Wnt reporter is undetectable in PHBCs (E-H′′,L-N′′) and occasional CtAs (N-N′′), but is present elsewhere. Detail zooms: Regions in white dashed boxes (A,E,I,L) shown in C-C′′,G-G′′,K-K′′ and N-N′′, respectively; regions in yellow dashed boxes (C,G) shown in D-D′′ and H-H′′, respectively. See also Fig. 8.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development