FIGURE

Fig. 7

ID

ZDB-FIG-150820-30

Publication

Westcot et al., 2015 - Protein-Trap Insertional Mutagenesis Uncovers New Genes Involved in Zebrafish Skin Development, Including a Neuregulin 2a-Based ErbB Signaling Pathway Required during Median Fin Fold Morphogenesis

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Fig. 7

MFF cleft cells of nrg2a mutants are largely unaffected, but ridge cells display altered morphology.

(A-C) Transverse sections through MFFs of Tg(Ola.Actb:Hsa.hras-egfp)vu119-expressing (hras-egfp) wild-type and nrg2a mutant embryos. Green: membrane-bound EGFP, blue: DAPI. (D-E) ET37 EGFP is expressed in MFF cleft cells (cc). ET37-EGFP: green, CellMask: red, DAPI: blue. (A) At 30 hpf, wild-type ridge cells (rc) are roughly cuboidal, with parallel apical and basal domains. The dermal space (ds) has not yet straightened, especially within the apical MFF terminus bounded by the cleft cell (cc). (B, D) By 52 hpf, the dermal space of wild-type embryos has straightened (arrowheads) and has invaginated into the basal side of the cleft cell. Ridge cells have elongated laterally, adopting a flat, planar, epithelial morphology. Their apical and basal domains are essentially parallel. (C, E) In nrg2a mutants, the cleft cell (cc) is present and contains the basal invagination of the dermal space (cleft; E) as in wild-type siblings. In contrast, nrg2a mutant ridge cells have elongated incorrectly and display an abnormal morphology, bulging basally into the dermal space, which acquires a serpentine-like appearance. Scale bar: 10 µm. Abbreviations: cc, cleft cell; rc, ridge cell; arrowheads point to dermal space.

Expression Data

Gene:	EGFP
Fish:	sqet37Et vu119Tg nrg2a^{mn0237Gt/mn0237Gt}; sqet37Et nrg2a^{mn0237Gt/mn0237Gt}; vu119Tg
Anatomical Terms:	apical ectodermal ridge median fin fold membrane cleft cell cleft cell membrane median fin fold membrane
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nrg2a^{mn0237Gt/mn0237Gt}; sqet37Et nrg2a^{mn0237Gt/mn0237Gt}; vu119Tg
Observed In:	apical ectodermal ridge median fin fold cell
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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