FIGURE

Fig. 2

ID

ZDB-FIG-150820-25

Publication

Westcot et al., 2015 - Protein-Trap Insertional Mutagenesis Uncovers New Genes Involved in Zebrafish Skin Development, Including a Neuregulin 2a-Based ErbB Signaling Pathway Required during Median Fin Fold Morphogenesis

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Fig. 2

GBT protein trapping identifies integument loci with epidermal or fin mesenchymal expression.

As identified by mRFP localization, seven GBT alleles have unique epidermal expression patterns emphasizing epidermal continuity over the body and larval fin folds. They are: (A, B) nrg2a^mn0237Gt, (C, D) col4a4^mn0275Gt, (E, F) arhgef25b^mn0175Gt, (G, H) ahnak^mn0196Gt, (I, J) capn12^mn0261Gt, (K, L) fkbp10b^mn0316Gt, and (M, N) msxc^mn0245Gt. (B, D, I, J, K) Four lines, nrg2a^mn0237Gt, col4a4^mn0275Gt, capn12^mn0261Gt, and fkbp10b^mn0316Gt, have “holes” or “gaps” in their epidermal pattern (green arrowheads) where neuromasts embedded in the basal layer exclude the mRFP-positive basal keratinocytes. (A, G, I, K, M) nrg2a^mn0237Gt (A), ahnak^mn0196Gt (G), capn12^mn0261Gt (I), fkbp10b^mn0316Gt (K), and msxc^mn0245Gt (M) are also expressed in the pectoral fin folds. (C-D, J, L, M-N) col4a4^mn0275Gt, capn12^mn0261Gt, fkbp10b^mn0316Gt, and msxc^mn0245Gt epidermal expression (blue arrowheads) can be difficult to discern among other expression pattern components. (I-N) capn12^mn0261Gt, megf6a^mn0316Gt, and msxc^mn0245Gt also show expression in fin mesenchymal cells (pink arrowheads). (C, D, K, L, M, N) Several lines are also expressed in other tissues. (C, D) col4a4^mn0275Gt appears in myotomes and the vascular plexus. (K, L) fkbp10b^mn0316Gt is seen in the notochord. (M, N) msxc^mn0245Gt is strongly expressed in the brain, spinal cord, and sensory maculae. hpf, hours post-fertilization. Comparisons of the mRFP localization patterns with the expression pattern of the endogenous genes are shown in Supporting Information (S1 Fig)

Expression Data

Gene:	mRFP1
Fish:	ahnak^mn0196Gt arhgef25b^mn0175Gt capn12^mn0261Gt col4a4^mn0275Gt fkbp10b^mn0316Gt msx3^mn0245Gt nrg2a^mn0237Gt
Anatomical Terms:	ball epidermis brain caudal vein plexus central nervous system epidermis extension epidermis eye epidermis fin fold pectoral fin bud epidermis head epidermis macula median fin fold epidermal cell mesenchyme median fin fold myotome notochord pectoral fin bud spinal cord
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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