FIGURE

Fig. 5

ID

ZDB-FIG-150609-11

Publication

Love et al., 2015 - Rest represses maturation within migrating facial branchiomotor neurons

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Fig. 5

Rest targets are inappropriately expressed in rest and pk1b mutants. In situ hybridization analysis of Rest target gene expression in Tg(islet1:GFP) embryos at 24 hpf in wild type, MZrest^sbu29/sbu29, and pk1b^fh122/fh122 specimens. Rest targets selected for analysis are adam23a (A–C′), snap25b (D–F′), srrm4 (G–I′), and syt4 (J–L′). We also analyzed the non-Rest target and FBMN maturation marker cmet (M–O2). All five genes are expressed in fully migrated FBMNs at 48 hpf. Upper panels show co-expression with islet1:GFP to label FBMNs, with gene expression alone shown below. At this stage (24 hpf), the selected Rest targets, as well as the maturation marker cmet, are not expressed in wild type FBMNs, but are expressed in both rest and pk1b mutant FBMNs (arrows).

Expression Data

Genes:	adam23a GFP met snap25b srrm4 syt4
Fish:	rw0Tg prickle1b^fh122/fh122; rw0Tg rest^sbu29/sbu29; rw0Tg
Anatomical Term:	facial nerve motor nucleus motor neuron
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	prickle1b^fh122/fh122; rw0Tg rest^sbu29/sbu29; rw0Tg
Observed In:	facial nerve motor nucleus motor neuron facial nerve motor nucleus motor neuron migration rhombomere 4 rhombomere 5 rhombomere 6
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 401(2), Love, C.E., Prince, V.E., Rest represses maturation within migrating facial branchiomotor neurons, 220-35, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.