ZFIN Image: Love et al., 2015, Fig. 5

Image

Figure Caption

Fig. 5

Rest targets are inappropriately expressed in rest and pk1b mutants. In situ hybridization analysis of Rest target gene expression in Tg(islet1:GFP) embryos at 24 hpf in wild type, MZrest^sbu29/sbu29, and pk1b^fh122/fh122 specimens. Rest targets selected for analysis are adam23a (A–C′), snap25b (D–F′), srrm4 (G–I′), and syt4 (J–L′). We also analyzed the non-Rest target and FBMN maturation marker cmet (M–O2). All five genes are expressed in fully migrated FBMNs at 48 hpf. Upper panels show co-expression with islet1:GFP to label FBMNs, with gene expression alone shown below. At this stage (24 hpf), the selected Rest targets, as well as the maturation marker cmet, are not expressed in wild type FBMNs, but are expressed in both rest and pk1b mutant FBMNs (arrows).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 401(2), Love, C.E., Prince, V.E., Rest represses maturation within migrating facial branchiomotor neurons, 220-35, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.