FIGURE

Fig. 4

ID

ZDB-FIG-120216-65

Publication

Yoruk et al., 2012 - Ccm3 functions in a manner distinct from Ccm1 and Ccm2 in a zebrafish model of CCM vascular disease

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Fig. 4

Morpholino knockdown of the GCKIII gene stk25b results in heart and vasculature defects similar to those seen in ccm3a/b morphants. (A) Confocal z-stack projections of dorsal head vasculature of stk25a and stk25b morphants at 54 hpf. Injection of stk25a morpholino (2 ng) caused misconnections in cranial vasculature; however, no appreciable vessel enlargements were observed. Injection of stk25b morpholino (6 ng) caused dilation of prosencephalic arteries (PrA), anterior cerebral veins (AceV) and severe mispatterning of the mesencephalic veins (MsV) in over 75% of the embryos imaged (yellow arrows, n > 80). (B) Light images of stk25a and stk25b morphants. stk25a morphants displayed a pericardial edema, severe yolk extension and trunk elongation defects. The stk25b morphant phenotype was more similar to ccm3a/b morphant morphology, with pericardial and venous plexus edemas.

Expression Data

Gene:	EGFP
Fish:	s843Tg s843Tg + MO2-stk25a s843Tg + MO3-stk25b
Knockdown Reagents:	MO2-stk25a MO3-stk25b
Anatomical Term:	cranial vasculature
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-stk25a WT + MO3-stk25b s843Tg + MO2-stk25a s843Tg + MO3-stk25b
Knockdown Reagents:	MO2-stk25a MO3-stk25b
Observed In:	anterior cerebral vein axis elongation caudal vein plexus cranial vasculature extension mesencephalic vein pericardium post-vent region prosencephalic artery
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 362(2), Yoruk, B., Gillers, B.S., Chi, N.C., and Scott, I.C., Ccm3 functions in a manner distinct from Ccm1 and Ccm2 in a zebrafish model of CCM vascular disease, 121-131, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.