ZFIN Image: Yoruk et al., 2012, Fig. 4

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Figure Caption

Fig. 4

Morpholino knockdown of the GCKIII gene stk25b results in heart and vasculature defects similar to those seen in ccm3a/b morphants. (A) Confocal z-stack projections of dorsal head vasculature of stk25a and stk25b morphants at 54 hpf. Injection of stk25a morpholino (2 ng) caused misconnections in cranial vasculature; however, no appreciable vessel enlargements were observed. Injection of stk25b morpholino (6 ng) caused dilation of prosencephalic arteries (PrA), anterior cerebral veins (AceV) and severe mispatterning of the mesencephalic veins (MsV) in over 75% of the embryos imaged (yellow arrows, n > 80). (B) Light images of stk25a and stk25b morphants. stk25a morphants displayed a pericardial edema, severe yolk extension and trunk elongation defects. The stk25b morphant phenotype was more similar to ccm3a/b morphant morphology, with pericardial and venous plexus edemas.

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Acknowledgments

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Reprinted from Developmental Biology, 362(2), Yoruk, B., Gillers, B.S., Chi, N.C., and Scott, I.C., Ccm3 functions in a manner distinct from Ccm1 and Ccm2 in a zebrafish model of CCM vascular disease, 121-131, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.