FIGURE

Fig. 3

ID

ZDB-FIG-120127-4

Publication

Wong et al., 2012 - Hedgehog signaling is required for differentiation of endocardial progenitors in zebrafish

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Fig. 3

Endocardial and myocardial markers are reduced in shh + twhh and smo MO knockdown and mutant embryos. Ventral view of the anterior region in flat-mounted embryos except as noted; anterior is to the top. (A–H) Injection of a mixture of shha and twhh/shhb MOs resulted in strong downregulation of endocardial kdrl (A,B,E,F), NFATc1 (G,H) (arrows) and myocardial cmlc2 (C,D) expression. (I–P) Smo MO injection results in the reduction or absence of kdrl-expressing endocardial progenitors at the midline at the 18-somite stage (arrows, I,J), the absence of endocardial kdrl (arrows, M,N) and NFATc1 expression at 26 hpf (arrows, O,P) and reduction of myocardial cmlc2 expression at the 20-somite stage (K,L). (Q–T) Smo-/- (R) and shha-/- (T) genetic mutant embryos display reduced endocardial NFATc1 expression (arrows) compared to their siblings (Q,S).

Expression Data

Genes:	kdrl myl7 nfatc1
Fish:	WT WT + MO4-smo WT + MO1-shha + MO1-shhb
Knockdown Reagents:	MO1-shha MO1-shhb MO4-smo
Anatomical Terms:	endocardium myocardium
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 361(2), Wong, K.S., Rehn, K., Palencia-Desai, S., Kohli, V., Hunter, W., Uhl, J.D., Rost, M.S., and Sumanas, S., Hedgehog signaling is required for differentiation of endocardial progenitors in zebrafish, 377-91, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.