Fig. 8
- ID
- ZDB-FIG-120127-9
- Publication
- Wong et al., 2012 - Hedgehog signaling is required for differentiation of endocardial progenitors in zebrafish
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Proliferation of endocardial progenitors is reduced in CyA-treated embryos compared to DMSO-treated controls. Maximum projection images of Tg(etsrp:EGFP) fluorescence (left), phospho-histone staining (right) and overlap of the two channels (middle) are shown. Ventral views of flattened deyolked embryos at the 13-somite stage, anterior is to the top. Formation of the midbrain-organizing center (MOC, arrows) which gives rise to many cranial endothelial, endocardial and myeloid progenitors is not significantly affected in CyA-treated embryos. However, phospho-histone staining within the MOC region (arrowheads) is significantly reduced in CyA-treated embryos. Note that because the presented view is the maximum projection image, staining in some cells may appear to overlap while they are actually present in different focal planes. Left graph shows average cell count per embryo of phospho-histone stained Tg(etsrp:EGFP) cells in the MOC region which is significantly different between DMSO and CyA-treated embryos. As shown in the right graph, the number of neural progenitors scored in a randomly selected area is not significantly different between the DMSO and CyA-treated embryos. |
Reprinted from Developmental Biology, 361(2), Wong, K.S., Rehn, K., Palencia-Desai, S., Kohli, V., Hunter, W., Uhl, J.D., Rost, M.S., and Sumanas, S., Hedgehog signaling is required for differentiation of endocardial progenitors in zebrafish, 377-91, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.