FIGURE

Fig. 4

ID

ZDB-FIG-101208-40

Publication

Schneider et al., 2010 - Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Asymmetric charon expression around the KV is dependent on Nkd1 and cilia function. Expression of charon in wt (A), CtrlMO^DFC (B), and Nkd1MO^DFC (C) embryos. Expression of charon in InvsMO (D), SeaMO (E), and Ift88MO (F) injected embryos. Dashed line represents embryonic midline. (G) Graph of percent of embryos displaying left-sided (white bar), no bias (shaded bar) or right-sided biased (black bar) charon expression around KV. (n) notes the sample size for each injection set. ** = p > 0.01 compared to wt using Fisher′s exact test. Nkd1MO^DFC p-value = 3.07 × 10^{- 16}; Nkd1MO^DFC+ nkd1 RNA p-value = 0.01, SeaMO p-value = 2.3 × 10^{- 11}; Ift88MO p-value = 3.5 × 10^{- 9}; InvsMO p-value = 2.11 × 10^{- 8}.

Expression Data

Gene:	dand5
Fish:	WT WT + MO3-dnaaf11 WT + MO3-nkd1 WT + MO4-ift88 WT + MO4-invs
Knockdown Reagents:	MO3-dnaaf11 MO3-nkd1 MO4-ift88 MO4-invs
Anatomical Term:	Kupffer's vesicle anatomical region
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-dnaaf11 WT + MO3-nkd1 WT + MO4-ift88 WT + MO4-invs
Knockdown Reagents:	MO3-dnaaf11 MO3-nkd1 MO4-ift88 MO4-invs
Observed In:	left/right pattern formation
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 348(1), Schneider, I., Schneider, P.N., Derry, S.W., Lin, S., Barton, L.J., Westfall, T., and Slusarski, D.C., Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning, 22-33, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.