FIGURE

Fig. 2

ID

ZDB-FIG-101208-38

Publication

Schneider et al., 2010 - Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning

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Fig. 2

Heart laterality defects after Nkd1 knockdown in DFCs. (A) RT-PCR using cDNA from wt or Nkd1MO-injected embryos: β-actin or Nkd1 were amplified. (B and C) Arrows denote efficient targeting of CtrlMO and Nkd1MO into the DFCs. (D–F) Bright field image of KV in wt (D), CtrlMO^DFC (E) and Nkd1MO^DFC(F) embryos, at 10-somite stage. Arrows indicate vesicle location; n = notochord. (G) Summary of KV morphology defects. (H–K) Arrows denote expression of nkx2.5 in the heart, on the left side in wt (H) and on the middle (I), right (J) and bilaterally (K) in Nkd1MO^DFC embryos. (L) Summary of cardiac jogging defects.

Expression Data

Gene:	nkx2.5
Fish:	WT + MO3-nkd1
Knockdown Reagent:	MO3-nkd1
Anatomical Term:	heart tube
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-nkd3 WT + MO2-nkd2a WT + MO3-nkd1
Knockdown Reagents:	MO1-nkd3 MO2-nkd2a MO3-nkd1
Observed In:	heart jogging heart tube Kupffer's vesicle left/right pattern formation
Stage Range:	10-13 somites to Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 348(1), Schneider, I., Schneider, P.N., Derry, S.W., Lin, S., Barton, L.J., Westfall, T., and Slusarski, D.C., Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning, 22-33, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.