FIGURE

Fig. 7

ID

ZDB-FIG-101208-43

Publication

Schneider et al., 2010 - Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning

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Fig. 7

Nkd1 knockdown promotes nuclear β-catenin accumulation in DFCs and activates β-catenin transcriptional targets. Immunohistochemistry on embryos at 80% epiboly. (A) The DFC region (dashed lines) was identified by the position relative to the gsc-GFP positive region (green) and yolk, cell membrane is noted by E-cadherin distribution. (B) In wt DFC, β-catenin is detected mostly in plasma membrane. (C) In Nkd1MO^DFC embryos, β-catenin is detected at the plasma membrane and in the nuclei of cells in DFC cluster. (D) Summary of β-catenin-positive nuclei present in DFCs of wt and Nkd1MO^DFC embryos. n = notochord. *P < 6.8 × 10^{- 6}, Fisher′s exact test. WMISH of axin2, dorsal side shown. (E) axin2 expression is excluded from the DFC region (arrow) with distinct lateral domains (asterisks) in ControlMO^DFC embryos. (F) Expanded axin2 expression is detected in Nkd1MO^DFC embryos. 91% show expanded domains, n = 33 compared to 7% in control MO, n = 27 (p-value = 2.1 × 10^{- 11}).

Expression Data

Gene:	axin2
Fish:	WT WT + MO3-nkd1
Knockdown Reagent:	MO3-nkd1
Anatomical Term:	whole organism
Stage:	90%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 348(1), Schneider, I., Schneider, P.N., Derry, S.W., Lin, S., Barton, L.J., Westfall, T., and Slusarski, D.C., Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning, 22-33, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.