FIGURE

Fig. 3

ID

ZDB-FIG-100802-37

Publication

Gao et al., 2010 - Oda16/Wdr69 is essential for axonemal dynein assembly and ciliary motility during zebrafish embryogenesis

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Fig. 3

Wdr69 is important for the establishment of left-right asymmetry. A-F: Analysis of organ left-right asymmetry by RNA in situ hybridizations using the heart marker cmlc2 (A-C) and gut marker foxa3 (D-F). Control morphants showed normal rightward looping of the heart (arrow in A) and left-sided orientation of the liver (arrow in D). In wdr69 MO^AUG morphants, heart looping was often reversed (arrow in B) or linear along the midline (arrow in C). Similarly, liver position in wdr69 MO^AUG morphants was frequently reversed (arrow in E) or bilateral (arrows in F). G-I: RNA in situ hybridizations detecting southpaw (spaw) expression in lateral plate mesoderm (LPM) at 18 somite stage (SS; G). spaw expression was restricted to left LPM cells in control morphants (arrow in G), whereas wdr69 MO^AUG morphants displayed abnormal right (arrow in H) and bilateral (arrows in I) distributions of spaw expression. J: The percentage of embryos injected with control MO (n = 130), wdr69 MO^AUG (n = 67) or wdr69 MO^I2E3 (n = 75) showing laterality defects in the heart and gut (see also Table 1). MO, morpholino oligonucleotides (MO).

Expression Data

Genes:	foxa3 myl7 spaw
Fish:	AB AB + MO1-daw1
Knockdown Reagent:	MO1-daw1
Anatomical Terms:	heart tube lateral plate mesoderm left side lateral plate mesoderm right side liver
Stage Range:	14-19 somites to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-daw1
Knockdown Reagent:	MO1-daw1
Observed In:	determination of left/right asymmetry in lateral mesoderm determination of liver left/right asymmetry heart looping heart tube liver
Stage Range:	14-19 somites to Prim-25

Phenotype Detail

Acknowledgments

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