Fig. 3

Fig. 3 Wdr69 is important for the establishment of left-right asymmetry. A-F: Analysis of organ left-right asymmetry by RNA in situ hybridizations using the heart marker cmlc2 (A-C) and gut marker foxa3 (D-F). Control morphants showed normal rightward looping of the heart (arrow in A) and left-sided orientation of the liver (arrow in D). In wdr69 MO^AUG morphants, heart looping was often reversed (arrow in B) or linear along the midline (arrow in C). Similarly, liver position in wdr69 MO^AUG morphants was frequently reversed (arrow in E) or bilateral (arrows in F). G-I: RNA in situ hybridizations detecting southpaw (spaw) expression in lateral plate mesoderm (LPM) at 18 somite stage (SS; G). spaw expression was restricted to left LPM cells in control morphants (arrow in G), whereas wdr69 MO^AUG morphants displayed abnormal right (arrow in H) and bilateral (arrows in I) distributions of spaw expression. J: The percentage of embryos injected with control MO (n = 130), wdr69 MO^AUG (n = 67) or wdr69 MO^I2E3 (n = 75) showing laterality defects in the heart and gut (see also Table 1). MO, morpholino oligonucleotides (MO).