FIGURE

Fig. 4

ID

ZDB-FIG-100802-38

Publication

Gao et al., 2010 - Oda16/Wdr69 is essential for axonemal dynein assembly and ciliary motility during zebrafish embryogenesis

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Fig. 4

Cilia length and number is not changed in wdr69 morphants. A-F: Cilia were visualized by immunofluorescent staining with acetylated tubulin antibodies. Cilia in embryos injected with wdr69 MO^AUG appeared similar to cilia injected with control MO in Kupffer′s vesicle (KV; A,B), pronephric ducts (C,D) and otic vesicles (arrows in E,F point to motile tether cilia). All red scale bars represent 10 μM. G,H: Measurements of KV cilia at six to eight somite stage (SS) showed no significant differences in the average length (G) or number (H) between wdr69 MO^AUG (n = 17) and control (n = 21) morphants. Error bars = one standard deviation. MO, morpholino oligonucleotides.

Expression Data

Antibody:	Ab1-tuba
Fish:	AB AB + MO1-daw1
Knockdown Reagent:	MO1-daw1
Anatomical Terms:	Kupffer's vesicle cilium otic vesicle cilium pronephric duct cilium
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-daw1
Knockdown Reagent:	MO1-daw1
Observed In:	Kupffer's vesicle cilium otic vesicle cilium pronephric duct cilium
Stage Range:	5-9 somites to 14-19 somites

Phenotype Detail

Acknowledgments

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