Zebrafish wdr69 is expressed in cells that assemble motile cilia. A: Protein sequence alignment of Oda16 homologs from Chlamydomonas reinhardtii, Danio renio, and Homo sapiens. Yellow color highlights conserved residues among the three species, blue color highlights residues conserved in two species and green color highlights conservative replacements. B-G: RNA in situ hybridizations detecting wdr69 expression during early zebrafish development. B,C: At the 80% (B) and 95% (C) epiboly stages, wdr69 is expressed exclusively in dorsal forerunner cells (DFCs). D,E: At the six somite stage (SS), wdr69 is expressed in Kupffer′s vesicle (KV) (D, E) and the floorplate (fp) of the neural tube (E). F,G: At 18 SS, wdr69 is expressed in the spinal cord (sc), pronephric ducts (pnd) (F) and otic vesicle (ov) (G).
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