FIGURE

Fig. 3

ID

ZDB-FIG-081103-40

Publication

Leung et al., 2008 - The orphan G protein-coupled receptor 161 is required for left-right patterning

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Fig. 3

gpr161 knockdown disrupts L–R identity in lateral plate mesoderm. (A) lefty2 expression in the left anterior lateral plate mesoderm (LPM) and lefty1 expression in the left diencephalon were disrupted in gpr161 knockdown embryos. (B) Graphical summary of control (99.3 ± 1.1%; n = 351), gpr161 morpholino (MO#24, 67.0 ± 4.7%; n = 348) and t-test (p < 0.01) results were from 11 injection experiments. (C) spaw expression in the left anterior lateral plate mesoderm (LPM) at 19.5 hpf in control and gpr161 knockdown embryos. ntl expression marked the midline as a reference. (D) Graphical summary of spaw expression in control (98.7 ± 1.4%; n = 311), gpr161 morpholino (MO#24, 63.4 ± 2.7%; n = 306) and t-test (p < 0.01) results were from 13 injection experiments. Error bars were ± SEM. All scale bars were 100 μm.

Expression Data

Genes:	lft1 lft2 spaw tbxta
Fish:	WT WT + MO1-gpr161b
Knockdown Reagent:	MO1-gpr161b
Anatomical Terms:	anterior lateral plate mesoderm diencephalon notochord
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-gpr161b
Knockdown Reagent:	MO1-gpr161b
Observed In:	anterior lateral plate mesoderm determination of left/right symmetry diencephalon
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 323(1), Leung, T., Humbert, J.E., Stauffer, A.M., Giger, K.E., Chen, H., Tsai, H.J., Wang, C., Mirshahi, T., and Robishaw, J.D., The orphan G protein-coupled receptor 161 is required for left-right patterning, 31-40, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.