FIGURE

Fig. S1

ID

ZDB-FIG-080516-23

Publication

Shin et al., 2008 - Multiple roles for Med12 in vertebrate endoderm development

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

shr is required for liver development. Wild-type and shr^s435 mutant animals at 34 (A, A′), 52 (B, B′), and 72 (C, C′) hpf were analyzed for hhex (A, A′) and sepp1b (B, B′) expression and confocal projections of pan-Cadherin (red) and Islet (blue) expression (C, C′). (A, A′) prox1 expression is evident in the liver (white arrow) of wild-type embryos but barely detectable in the liver region of shr^s435 mutants. (B, B′) selenoprotein P, plasma, 1b (sepp1b) expression is detected at low levels in shr^s435 mutants (black arrow). (C, C′) shr^s435 mutants exhibit defective differentiation of hepatocytes as assessed by Cadherin immunostaining. Note the multiple, unfused ventral pancreatic buds in mutants (white arrowheads). Yellow arrow points to the liver. A–B′ are dorsal views, anterior to the left; C, C′ are ventral views, anterior to the top.

Expression Data

Genes:	prox1a selenop2
Antibodies:	Ab1-pan-Cadherin Ab2-isl
Fish:	WT med12^s435/s435
Anatomical Terms:	hepatocyte liver pancreas
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	med12^s435/s435
Observed In:	endodermal cell fate specification liver liver development pancreas
Stage Range:	Prim-15 to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 317(2), Shin, C.H., Chung, W.S., Hong, S.K., Ober, E.A., Verkade, H., Field, H.A., Huisken, J., and Stainier, D.Y., Multiple roles for Med12 in vertebrate endoderm development, 467-479, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.