Fig. 4

Med12 does not appear to be essential for embryonic cell proliferation or viability. (A–B′) Confocal projections of wild-type and med12^s435 mutant embryos in the Tg(gutGFP)^s854 line (green) at 34 (A, A′) and 48 (B, B′) hpf in conjunction with BrdU (red) antibody staining. Yellow cells are endodermal cells (green) with BrdU (red) incorporation and white dashed lines surround the area used to count BrdU-positive cells. The overall level of endodermal BrdU incorporation in wild-type and med12^s435mutant embryos appears similar (Table 1). (C–D′) Confocal projections of wild-type host embryos transplanted with wild-type (C, D) or med12^s435 mutant (C′, D′) donor cells, visualized at days 5 (C, C′) and 12 (D, D′). Most wild-type and med12^s435mutant donor cells survive until at least day 12 (D, D′). White arrowheads point to individual rhodamine dextran-labeled donor cells. The same embryo is shown in panels C and D, and another in panels C′ and D′. Images A–B′ are ventral views, anterior to the top; C–D′ are dorsal views, anterior to the top.