FIGURE

Fig. S3

ID

ZDB-FIG-071227-24

Publication

Esguerra et al., 2007 - Ttrap is an essential modulator of Smad3-dependent Nodal signaling during zebrafish gastrulation and left-right axis determination

Other Figures

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Fig. S3

Test for functionality and expression of mutant and wild-type human TTRAP in zebrafish embryos. (A) Co-IP of Smad3 with TTRAP or TTRAP^T88A,^T92A. TTRAP-pCS3 or TTRAP^T88A,^T92A-pCS3 were co-expressed with HA-tagged Smad3 or Flag-tagged TTRAP-frame-shift as control in HEK293T cells and co-immunoprecipitated using anti-HA antibody. The precipitates were immunoblotted and proteins detected with monoclonal anti-HA (middle panel) or monoclonal anti-TTRAP antibody (top and bottom panel). (B) Western blot analysis of TTRAP and TTRAP^T88A,^T92A expression in zebrafish embryos. Total protein of zebrafish embryos injected with TTRAP-pCS2 or TTRAP^T88A,^T92A-pCS2 mRNA was isolated 2 hours, 4 hours and 6 hours after injection and incubated with TTRAP antibody to check expression levels. An anti-tubulin antibody was used to control for input and loading.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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