FIGURE

Fig. S4

ID

ZDB-FIG-071227-25

Publication

Esguerra et al., 2007 - Ttrap is an essential modulator of Smad3-dependent Nodal signaling during zebrafish gastrulation and left-right axis determination

Other Figures

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Fig. S4

Targeting of eGFP mRNA to DFCs and expression in Kupffer’s Vesicle. (A-C, A′-C′) Live embryos at 7-somite stage. (A-C) Fluorescence images of eGFP^DFCOE embryos showing eGFP expression only in the yolk and KV and not in the rest of the embryo. (A′-C′) Corresponding transmitted light images of embryos in A-C. (B) Occasionally, eGFP could be found filling the entire KV (red arrowhead), whereas in the majority of embryos, such as in (C), eGFP only outlines part of the KV (red arrowhead), implying that only a subset of DFCs take up and/or express eGFP RNA/protein. Embryos in (B′ and C′) showing fully formed KV (black arrowheads). All eGFP^DFCOE embryos (n=160) developed normally. (A and A′) Lateral view. (B, B′, C, and C′) Posterior views.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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