ZFIN Image: Esguerra et al., 2007, Fig. S3

Image

Figure Caption

Fig. S3 Test for functionality and expression of mutant and wild-type human TTRAP in zebrafish embryos. (A) Co-IP of Smad3 with TTRAP or TTRAP^T88A,^T92A. TTRAP-pCS3 or TTRAP^T88A,^T92A-pCS3 were co-expressed with HA-tagged Smad3 or Flag-tagged TTRAP-frame-shift as control in HEK293T cells and co-immunoprecipitated using anti-HA antibody. The precipitates were immunoblotted and proteins detected with monoclonal anti-HA (middle panel) or monoclonal anti-TTRAP antibody (top and bottom panel). (B) Western blot analysis of TTRAP and TTRAP^T88A,^T92A expression in zebrafish embryos. Total protein of zebrafish embryos injected with TTRAP-pCS2 or TTRAP^T88A,^T92A-pCS2 mRNA was isolated 2 hours, 4 hours and 6 hours after injection and incubated with TTRAP antibody to check expression levels. An anti-tubulin antibody was used to control for input and loading.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development