FIGURE

Fig. S1

ID

ZDB-FIG-071227-23

Publication

Esguerra et al., 2007 - Ttrap is an essential modulator of Smad3-dependent Nodal signaling during zebrafish gastrulation and left-right axis determination

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Fig. S1

Translational inhibition of Ttrap protein by Ttrap MO. Western blot analysis of HA-tagged zebrafish Ttrap, either alone (left lane), after co-injection with scrambled control MO (SCMO) (middle lane), or after co-injection with TtrapMO (1:1 mix of Ttrap MO1 and Ttrap MO2 (16 ng total), as used throughout this study) (right lane). Since none of the available antibodies against mammalian Ttrap were capable of detecting zebrafish Ttrap in vivo, we resorted to mRNA overexpresssion of full-length, HA-tagged zebrafish Ttrap (zfTtrap-HA) in fish embryos. Western blot analysis using an anti-HA antibody resulted in a single band of 55 kDa which is clearly downregulated in Ttrap^MO embryos. An anti-tubulin antibody was used to control for input and loading.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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