ZFIN Image: Esguerra et al., 2007, Fig. S1

Image

Figure Caption

Fig. S1 Translational inhibition of Ttrap protein by Ttrap MO. Western blot analysis of HA-tagged zebrafish Ttrap, either alone (left lane), after co-injection with scrambled control MO (SCMO) (middle lane), or after co-injection with TtrapMO (1:1 mix of Ttrap MO1 and Ttrap MO2 (16 ng total), as used throughout this study) (right lane). Since none of the available antibodies against mammalian Ttrap were capable of detecting zebrafish Ttrap in vivo, we resorted to mRNA overexpresssion of full-length, HA-tagged zebrafish Ttrap (zfTtrap-HA) in fish embryos. Western blot analysis using an anti-HA antibody resulted in a single band of 55 kDa which is clearly downregulated in Ttrap^MO embryos. An anti-tubulin antibody was used to control for input and loading.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development