FIGURE

Fig. 2

ID

ZDB-FIG-071219-2

Publication

Gardiner et al., 2007 - A global role for zebrafish klf4 in embryonic erythropoiesis

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Fig. 2

A global role for klf4 in embryonic erythropoiesis and hatching gland gene expression. (A) Scatter plot of mean relative expression (from four biological replicates) in klf4-MO injected embryos (y-axis) versus Std-MO injected embryos (x-axis). Lines representing 1.4-fold up and down regulation are shown. A few selected differentially expressed genes are indicated. (B–E) Quantitative real time-PCR validation of gene expression for catL, he1, βe3-globin, gata1, alas2, βa1-globin, runx1, c-myb, gata2, and scl at 20 hpf (B), 24 hpf (C), 36 hpf (D) and 48 hpf (E). Values are determined from the delta CT value and expressed relative to expression of γ-actin. Values are means of four experiments and are expressed as log relative expression of klf4-MO divided by Std-MO.

Expression Data

Genes:	alas2 ctslb gata1a gata2a hbbe3 myb runx1 tal1
Fish:	WT WT + MO2-klf17
Knockdown Reagent:	MO2-klf17
Anatomical Terms:	unspecified whole organism
Stage Range:	20-25 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 124(9-10), Gardiner, M.R., Gongora, M.M., Grimmond, S.M., and Perkins, A.C., A global role for zebrafish klf4 in embryonic erythropoiesis, 762-774, Copyright (2007) with permission from Elsevier. Full text @ Mech. Dev.